MA

Mahbube Jafari


Education:


2015 – 2018   M.Sc. in Human Genetics



  • Shahrekord University of Medical Sciences, Shahrekord, Iran
  • Thesis Topic: “Sh-RNA Mediated NAGA Gene Silencing and Evaluation of the Effects on TNFR2 and
    a2b1 
    Integrin Signaling Pathways in Cancer Cell Lines”
  • Supervisor: Prof. Morteza Hashemzadeh Chaleshtori, Dr. Seyed Abbas Mirzaei
  • GPA:  18.41/20

2010 – 2014   B.Sc. in Genetics



  • University of Shahrekord, Shahrekord, Iran
  • GPA:  17.59/20

Work Experience


· Position: Research assistant      July 2019-present


· Location: A. Immunodeficiency Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran


· Project Title: Preparation of Genetic Database for PID Patients


Publications



  • Synergistic Effects of Lauryl Gallate and Tamoxifen on Human Breast Cancer Cells, IJPM (forthcoming)
  • shRNA-mediated Downregulation of α-N-Acetylgalactosaminidase Can Affect Cancer-Related Cellular Signaling Pathways (in progress)

Laboratory Skills



  • Lab Safety Principles (GLP)
  • Polymerase Chain Reaction (PCR)
  • Gel Electrophoresis
  • Real-time Polymerase Chain Reaction
  • Cloning
  • Cell Culture
  • Transfection
  • Fluorescent & Light Microscopy
  • Western Blotting
  • Flow cytometry


  • Karyotyping


  • Bioinformatics

Bioinformatic Skills



  • Bioinformatics and genomics databases (NCBI, Ensembl, UniProt, and EMBL-EBI database)
  • Primer designing


  • siRNA/shRNA designing


  • Applications: Microsoft office package, Gene Runner, Rest, Vector NTI, ImageJ, GraphPad Prism, SPSS, Endnote, Mendeley

Research Interests



  • Human Genetics
  • Cancer Genetics
  • Immunogenetics
  • Bioinformatics and Genomics (Next Generation Sequencing Technologies)
  • Gene Therapy & Gene Editing
  • Cytogenetics
  • Genetic and Epigenetic Related Disease
  • Gene Expression Analysis at Transcription Level (Transcriptomics)
  • Gene Expression Analysis at Translation Level (Proteomics)
  • Non-Coding RNAs & miRNAs

Honors



  • B.Sc. Ranked 3rd among 40 bachelor students as an exceptionally talented student, Shahrekord University, Shahrekord, Iran
  • M.Sc. Talented student, Shahrekord University of Medical Sciences, Shahrekord, Iran
  • Ranked 1st in seminar presentation among M.Sc. and Ph.D. students, with the title of “The Role of α-NaGalase Enzyme in Cancer Progression”, 2016,
    Cellular and molecular research center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Seminar & Congresses



  • 2nd International and 14th Iranian Genetics Congress, Tehran, Iran. May 21-23 2016


  • Participation in “Non-coding RNAs and Diseases” Symposium, Shahrekord, Iran, November 26, 2017


  • Poster presentation as first author entitled "Synergistic Effects of Lauryl Gallate and Tamoxifen on Human breast Cancer Cell", 3rd International and 15th Iranian Genetics Congress, Tehran, Iran. May 13-15 2018


  • Poster presentation as the first author entitled “The Role of α–N-acetylgalactosaminidase enzyme in EPG 85/257 RDB Cell Line Signaling Pathways”,
    6th International and 15th Iranian International Congress of Biochemistry and Molecular Biology, Isfahan, Iran. August 25-28 2018


  • Two days of PCR-SSCP method workshop, 2015, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
  • Five days of Molecular diagnostic methods workshop, 2015, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
  • The cytogenetic three-day workshop, 2015, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
  • Three days of Cloning workshop, 2016, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
  • Two-day Western Blot workshop, 2017, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran
  • Two days of computational biology workshop, 2017, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran
  • Two-day SPSS workshop, 2018, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran

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