Mahbube Jafari
Education:
2015 – 2018 M.Sc. in Human Genetics
- Shahrekord University of Medical Sciences, Shahrekord, Iran
- Thesis Topic: “Sh-RNA Mediated NAGA Gene Silencing and Evaluation of the Effects on TNFR2 and
a2b1
Integrin Signaling Pathways in Cancer Cell Lines” - Supervisor: Prof. Morteza Hashemzadeh Chaleshtori, Dr. Seyed Abbas Mirzaei
- GPA: 18.41/20
2010 – 2014 B.Sc. in Genetics
- University of Shahrekord, Shahrekord, Iran
- GPA: 17.59/20
Work Experience
· Position: Research assistant July 2019-present
· Location: A. Immunodeficiency Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
· Project Title: Preparation of Genetic Database for PID Patients
Publications
- Synergistic Effects of Lauryl Gallate and Tamoxifen on Human Breast Cancer Cells, IJPM (forthcoming)
- shRNA-mediated Downregulation of α-N-Acetylgalactosaminidase Can Affect Cancer-Related Cellular Signaling Pathways (in progress)
Laboratory Skills
- Lab Safety Principles (GLP)
- Polymerase Chain Reaction (PCR)
- Gel Electrophoresis
- Real-time Polymerase Chain Reaction
- Cloning
- Cell Culture
- Transfection
- Fluorescent & Light Microscopy
- Western Blotting
- Flow cytometry
- Karyotyping
- Bioinformatics
Bioinformatic Skills
- Bioinformatics and genomics databases (NCBI, Ensembl, UniProt, and EMBL-EBI database)
- Primer designing
- siRNA/shRNA designing
- Applications: Microsoft office package, Gene Runner, Rest, Vector NTI, ImageJ, GraphPad Prism, SPSS, Endnote, Mendeley
Research Interests
- Human Genetics
- Cancer Genetics
- Immunogenetics
- Bioinformatics and Genomics (Next Generation Sequencing Technologies)
- Gene Therapy & Gene Editing
- Cytogenetics
- Genetic and Epigenetic Related Disease
- Gene Expression Analysis at Transcription Level (Transcriptomics)
- Gene Expression Analysis at Translation Level (Proteomics)
- Non-Coding RNAs & miRNAs
Honors
- B.Sc. Ranked 3rd among 40 bachelor students as an exceptionally talented student, Shahrekord University, Shahrekord, Iran
- M.Sc. Talented student, Shahrekord University of Medical Sciences, Shahrekord, Iran
- Ranked 1st in seminar presentation among M.Sc. and Ph.D. students, with the title of “The Role of α-NaGalase Enzyme in Cancer Progression”, 2016,
Cellular and molecular research center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Seminar & Congresses
- 2nd International and 14th Iranian Genetics Congress, Tehran, Iran. May 21-23 2016
- Participation in “Non-coding RNAs and Diseases” Symposium, Shahrekord, Iran, November 26, 2017
- Poster presentation as first author entitled "Synergistic Effects of Lauryl Gallate and Tamoxifen on Human breast Cancer Cell", 3rd International and 15th Iranian Genetics Congress, Tehran, Iran. May 13-15 2018
- Poster presentation as the first author entitled “The Role of α–N-acetylgalactosaminidase enzyme in EPG 85/257 RDB Cell Line Signaling Pathways”,
6th International and 15th Iranian International Congress of Biochemistry and Molecular Biology, Isfahan, Iran. August 25-28 2018
- Two days of PCR-SSCP method workshop, 2015, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
- Five days of Molecular diagnostic methods workshop, 2015, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
- The cytogenetic three-day workshop, 2015, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
- Three days of Cloning workshop, 2016, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
- Two-day Western Blot workshop, 2017, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran
- Two days of computational biology workshop, 2017, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran
- Two-day SPSS workshop, 2018, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran
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